Rizo lab

Tien, C.-W.,Yu, B., Huang, M., Stepien. K. P., Sugita, K., Xie, X., Han, L., Monnier, P. P., Zhen, M., Rizo, J.*, Gao, S.*, and Sugita, S.* Open syntaxin overcomes exocytosis defects of diverse mutants in C. elegans, Nat. Commun., in press.

 

Voleti, R., Jaczynska, K., and Rizo, J. (2020) Ca(2+)-dependent release of Synaptotagmin-1 from the SNARE complex on phosphatidylinositol 4,5-bisphosphate-containing membranes, Elife 9, e57154.

 

Magdziarek, M., Bolembach, A. A., Stepien, K. P., Quade, B., Liu, X., and Rizo, J. (2020) Re-examining how Munc13-1 facilitates opening of syntaxin-1, Protein Sci 29, 1440-1458.

 

Pan, Y. Z., Liu, X., and Rizo, J. (2020) Analysis of asymmetry in lipid and content mixing assays with reconstituted proteoliposomes containing the neuronal SNAREs, Sci Rep 10, 2907.

 

Stepien, K. P., Prinslow, E. A., and Rizo, J. (2019) Munc18-1 is crucial to overcome the inhibition of synaptic vesicle fusion by alphaSNAP, Nat Commun 10, 4326.

 

Quade, B., Camacho, M., Zhao, X., Orlando, M., Trimbuch, T., Xu, J., Li, W., Nicastro, D., Rosenmund, C. *, and Rizo, J.* (2019) Membrane bridging by Munc13-1 is crucial for neurotransmitter release, Elife 8, e42806.

 

Prinslow, E. A., Stepien, K. P., Pan, Y. Z., Xu, J., and Rizo, J. (2019) Multiple factors maintain assembled trans-SNARE complexes in the presence of NSF and alphaSNAP, Elife 8, e38880.

 

Rizo, J. (2018) Mechanism of neurotransmitter release coming into focus, Protein Sci 27, 1364-1391.

 

de Jong, A. P. H., Roggero, C. M., Ho, M. R., Wong, M. Y., Brautigam, C. A., Rizo, J., and Kaeser, P. S. (2018) RIM C2B Domains Target Presynaptic Active Zone Functions to PIP2-Containing Membranes, Neuron 98, 335-349 e337.

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Voleti, R., Tomchick, D. R., Sudhof, T. C.*, and Rizo, J.* (2017) Exceptionally tight membrane-binding may explain the key role of the synaptotagmin-7 C2A domain in asynchronous neurotransmitter release, Proc Natl Acad Sci U S A 114, E8518-E8527.

 

Prinslow, E. A., Brautigam, C. A., and Rizo, J. (2017) Reconciling isothermal titration calorimetry analyses of interactions between complexin and truncated SNARE complexes, Elife 6, e30286.

 

Sitarska, E., Xu, J., Park, S., Liu, X., Quade, B., Stepien, K., Sugita, K., Brautigam, C. A., Sugita, S., and Rizo, J. (2017) Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion, Elife 6, e24278.

 

Xu, J., Camacho, M., Xu, Y., Esser, V., Liu, X., Trimbuch, T., Pan, Y. Z., Ma, C., Tomchick, D. R. *, Rosenmund, C.*, and Rizo, J.* (2017) Mechanistic insights into neurotransmitter release and presynaptic plasticity from the crystal structure of Munc13-1 C1C2BMUN, Elife 6, e22567.

 

Liu, X., Seven, A. B., Camacho, M., Esser, V., Xu, J., Trimbuch, T., Quade, B., Su, L., Ma, C., Rosenmund, C., and Rizo, J. (2016) Functional synergy between the Munc13 C-terminal C1 and C2 domains, elife 5, e13696.

 

Pan, Y. Z., Quade, B., Brewer, K. D., Szabo, M., Swarbrick, J. D., Graham, B., and Rizo, J. (2016) Sequence-specific assignment of methyl groups from the neuronal SNARE complex using lanthanide-induced pseudocontact shifts, J Biomol NMR 66, 281-293.

 

Rizo, J., and Xu, J. (2015) The Synaptic Vesicle Release Machinery, Annu. Rev. Biophys 44, 339-367.

 

Brewer, K. D., Bacaj, T., Cavalli, A., Camilloni, C., Swarbrick, J. D., Liu, J., Zhou, A., Zhou, P., Barlow, N., Xu, J., Seven, A. B.,

Prinslow, E. A., Voleti, R., Haussinger, D., Bonvin, A. M., Tomchick, D. R., Vendruscolo, M., Graham, B., Sudhof, T. C., and Rizo, J. (2015) Dynamic binding mode of a Synaptotagmin-1-SNARE complex in solution, Nat. Struct. Mol. Biol 22, 555-564.

 

Mukherjee, S., Zheng, H., Derebe, M. G., Callenberg, K. M., Partch, C. L., Rollins, D., Propheter, D. C., Rizo, J., Grabe, M., Jiang, Q. X., and Hooper, L. V. (2014) Antibacterial membrane attack by a pore-forming intestinal C-type lectin, Nature 505, 103-107.

 

Wang, H., Sun, L., Su, L., Rizo, J., Liu, L., Wang, L. F., Wang, F. S., and Wang, X. (2014) Mixed lineage kinase domain-like protein MLKL causes necrotic membrane disruption upon phosphorylation by RIP3, Mol. Cell 54, 133-146.

 

Trimbuch, T., Xu, J., Flaherty, D., Tomchick, D. R., Rizo, J.*, and Rosenmund, C.* (2014) Re-examining how complexin inhibits neurotransmitter release, elife 3, e02391.

 

Xu, J., Bacaj, T., Zhou, A., Tomchick, D. R., Sudhof, T. C., and Rizo, J. (2014) Structure and Ca(2)(+)-binding properties of the tandem C(2) domains of E-Syt2, Structure 22, 269-280.

 

Su, L., Quade, B., Wang, H., Sun, L., Wang, X., and Rizo, J. (2014) A plug release mechanism for membrane permeation by MLKL, Structure 22, 1489-1500.

 

Ma, C. *, Su, L., Seven, A. B., Xu, Y., and Rizo, J.* (2013) Reconstitution of the vital functions of Munc18 and Munc13 in neurotransmitter release, Science 339, 421-425.

 

Rizo, J., and Sudhof, T. C. (2012) The Membrane Fusion Enigma: SNAREs, Sec1/Munc18 Proteins, and Their Accomplices-Guilty as Charged?, Annu. Rev. Cell Dev. Biol 28, 279-308.

 

Rizo, J. (2012) Cell biology. Staging membrane fusion, Science 337, 1300-1301.

 

Kaeser, P. S., Deng, L., Wang, Y., Dulubova, I., Liu, X., Rizo, J., and Sudhof, T. C. (2011) RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction, Cell 144, 282-295.

 

Ma, C., Li, W., Xu, Y., and Rizo, J. (2011) Munc13 mediates the transition from the closed syntaxin-Munc18 complex to the SNARE complex, Nat. Struct. Mol. Biol 18, 542-549.

 

Li, W., Ma, C., Guan, R., Xu, Y., Tomchick, D.R.*, and Rizo, J.* (2011) The crystal structure of a Munc13 C-terminal module exhibits a remarkable similarity to vesicle tethering factors. Structure 19, 1443-1455.

 

Sheard, L. B., Tan, X., Mao, H., Withers, J., Ben-Nissan, G., Hinds, T. R., Kobayashi, Y., Hsu, F. F., Sharon, M., Browse, J., He, S. Y., Rizo, J., Howe, G. A., and Zheng, N. (2010) Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor, Nature 468, 400-405.

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Shin, O. H., Lu, J., Rhee, J. S., Tomchick, D. R., Pang, Z. P., Wojcik, S. M., Camacho-Perez, M., Brose, N., Machius, M., Rizo, J. *, Rosenmund, C.*, and Sudhof, T. C.* (2010) Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis, Nat. Struct. Mol. Biol 17, 280-288.

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Xue, M., Craig, T. K., Xu, J., Chao, H. T., Rizo, J.*, and Rosenmund, C.* (2010) Binding of the complexin N terminus to the SNARE complex potentiates synaptic-vesicle fusogenicity, Nat. Struct. Mol. Biol 17, 568-575.

 

Deák. F., Xu, Y., Chang, W.-P., Dulubova, I., Khvotchev, M., Liu, X., Sudhof, T. C.* and Rizo, J.* (2009) Munc18-1 binding to the neuronal SNARE complex controls synaptic vesicle priming. J. Cell Biol. 184, 751-764.

 

Gerber, S. H., Rah, J. C., Min, S. W., Liu, X., de, W. H., Dulubova, I., Meyer, A. C., Rizo, J., Arancillo, M., Hammer, R. E., Verhage, M., Rosenmund, C., and Sudhof, T. C. (2008) Conformational switch of syntaxin-1 controls synaptic vesicle fusion, Science 321, 1507-1510.

 

Xue, M., Ma, C., Craig, T. K., Rosenmund, C.*, and Rizo, J.* (2008) The Janus-faced nature of the C(2)B domain is fundamental for synaptotagmin-1 function, Nat. Struct. Mol. Biol 15, 1160-1168.

 

Dulubova, I., Khvotchev, M., Liu, S., Huryeva, I., Sudhof, T. C.*, and Rizo, J.* (2007) Munc18-1 binds directly to the neuronal SNARE complex, Proc. Natl. Acad. Sci. U. S. A 104, 2697-2702.

 

Dai, H., Shen, N., Arac, D., and Rizo, J. (2007) A Quaternary SNARE-Synaptotagmin-Ca(2+)-Phospholipid Complex in Neurotransmitter Release, J. Mol. Biol 367, 848-863.

 

Yang, M., Li, B., Tomchick, D. R., Machius, M., Rizo, J., Yu, H., and Luo, X. (2007) p31comet blocks Mad2 activation through structural mimicry, Cell 131, 744-755.

 

Rizo, J. *, and Rosenmund, C. * (2008) Synaptic vesicle fusion, Nat. Struct. Mol. Biol 15, 665-674.

 

Arac, D., Chen, X., Khant, H. A., Ubach, J., Ludtke, S. J., Kikkawa, M., Johnson, A. E., Chiu, W., Sudhof, T. C., and Rizo, J. (2006) Close membrane-membrane proximity induced by Ca(2+)-dependent multivalent binding of synaptotagmin-1 to phospholipids, Nat. Struct. Mol. Biol 13, 209-217.

 

Lu, J., Machius, M., Dulubova, I., Dai, H., Sudhof, T. C., Tomchick, D. R., and Rizo, J. (2006) Structural Basis for a Munc13-1 Homodimer to Munc13-1/RIM Heterodimer Switch, PLoS. Biol 4, e192.

 

Tang, J., Maximov, A., Shin, O. H., Dai, H., Rizo, J., and Sudhof, T. C. (2006) A complexin/synaptotagmin 1 switch controls fast synaptic vesicle exocytosis, Cell 126, 1175-1187.

 

Rizo, J., Chen, X., and Arac, D. (2006) Unraveling the mechanisms of synaptotagmin and SNARE function in neurotransmitter release, Trends Cell Biol 16, 339-350.

 

Basu, J., Shen, N., Dulubova, I., Lu, J., Guan, R., Guryev, O., Grishin, N. V., Rosenmund, C., and Rizo, J. (2005) A minimal domain responsible for Munc13 activity, Nat. Struct. Mol. Biol 12, 1017-1018.

 

Dulubova, I., Lou, X., Lu, J., Huryeva, I., Alam, A., Schneggenburger, R., Sudhof, T. C., and Rizo, J. (2005) A Munc13/RIM/Rab3 tripartite complex: from priming to plasticity?, EMBO J 24, 2839-2850.

 

Garcia, J., Gerber, S. H., Sugita, S., Sudhof, T. C., and Rizo, J. (2004) A conformational switch in the Piccolo C2A domain regulated by alternative splicing, Nat. Struct. Mol. Biol 11, 45-53.

 

Dai, H., Shin, O. H., Machius, M., Tomchick, D. R., Sudhof, T. C., and Rizo, J. (2004) Structural basis for the evolutionary inactivation of Ca2+ binding to synaptotagmin 4, Nat. Struct. Mol. Biol 11, 844-849.

 

Luo, X., Tang, Z., Xia, G., Wassmann, K., Matsumoto, T., Rizo, J.*, and Yu, H.* (2004) The Mad2 spindle checkpoint protein has two distinct natively folded states, Nat. Struct. Mol. Biol 11, 338-345.

 

Chen, X., Tomchick, D. R., Kovrigin, E., Arac, D., Machius, M., Sudhof, T. C., and Rizo, J. (2002) Three-dimensional structure of the complexin/SNARE complex, Neuron 33, 397-409.

 

Yamaguchi, T., Dulubova, I., Min, S. W., Chen, X., Rizo, J., and Sudhof, T. C. (2002) Sly1 binds to Golgi and ER syntaxins via a conserved N-terminal peptide motif, Dev. Cell 2, 295-305.

 

Dulubova, I., Yamaguchi, T., Gao, Y., Min, S. W., Huryeva, I., Sudhof, T. C., and Rizo, J. (2002) How Tlg2p/syntaxin 16 'snares' Vps45, EMBO J 21, 3620-3631.

 

Luo, X., Tang, Z., Rizo, J.*, and Yu, H.* (2002) The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20, Mol. Cell 9, 59-71.

 

Fernandez-Chacon, R., Konigstorfer, A., Gerber, S. H., Garcia, J., Matos, M. F., Stevens, C. F., Brose, N., Rizo, J., Rosenmund, C., and Sudhof, T. C. (2001) Synaptotagmin I functions as a calcium regulator of release probability, Nature 410, 41-49.

 

Fernandez, I., Arac, D., Ubach, J., Gerber, S. H., Shin, O., Gao, Y., Anderson, R. G., Sudhof, T. C., and Rizo, J. (2001) Three-dimensional structure of the synaptotagmin 1 c(2)b-domain. Synaptotagmin 1 as a phospholipid binding machine, Neuron 32, 1057-1069.

 

Dulubova, I., Yamaguchi, T., Wang, Y., Sudhof, T. C., and Rizo, J. (2001) Vam3p structure reveals conserved and divergent properties of syntaxins, Nat. Struct. Biol 8, 258-264.

 

Dulubova, I., Sugita, S., Hill, S., Hosaka, M., Fernandez, I., Sudhof, T. C.*, and Rizo, J.* (1999) A conformational switch in syntaxin during exocytosis: role of munc18, EMBO J 18, 4372-4382.

 

Ubach, J., Garcia, J., Nittler, M. P., Sudhof, T. C., and Rizo, J. (1999) Structure of the Janus-faced C2B domain of rabphilin, Nat. Cell Biol 1, 106-112.

 

Shao, X., Fernandez, I., Sudhof, T. C., and Rizo, J. (1998) Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change?, Biochemistry 37, 16106-16115.

 

Zhang, X., Rizo, J., and Sudhof, T. C. (1998) Mechanism of phospholipid binding by the C2A-domain of synaptotagmin I, Biochemistry 37, 12395-12403.

 

Ubach, J., Zhang, X., Shao, X., Sudhof, T. C., and Rizo, J. (1998) Ca2+ binding to synaptotagmin: how many Ca2+ ions bind to the tip of a C2-domain?, EMBO J 17, 3921-3930.

 

Rizo, J.*, and Sudhof, T. C.* (1998) C2-domains, structure and function of a universal Ca2+-binding domain, J. Biol. Chem 273, 15879-15882.

 

Fernandez, I., Ubach, J., Dulubova, I., Zhang, X., Sudhof, T. C., and Rizo, J. (1998) Three-dimensional structure of an evolutionarily conserved N-terminal domain of syntaxin 1A, Cell 94, 841-849.

 

Shao, X., Li, C., Fernandez, I., Zhang, X., Sudhof, T. C., and Rizo, J. (1997) Synaptotagmin-syntaxin interaction: the C2 domain as a Ca2+-dependent electrostatic switch, Neuron 18, 133-142.

 

Shao, X., Davletov, B. A., Sutton, R. B., Sudhof, T. C., and Rizo, J. (1996) Bipartite Ca2+-binding motif in C2 domains of synaptotagmin and protein kinase C, Science 273, 248-251.

 

*Co-corresponding authors